碧云天生物技术-Quickblock封闭液(PBS)(P0220)

试剂> 多肽与蛋白> 蛋白检测> Western检测

QuickBlock™封闭液(PBS)

一键复制产品信息

产品编号: P0220

产品包装:100ml

选择包装

100ml

数

量

说明书下载

价格: ¥ 123.00

加入购物车

				产品简介
			
				使用说明
			
				产品文件
			
				相关产品
			
				相关论文
			
				产品问答

产品编号	产品名称	产品包装	产品价格
P0220	QuickBlock™封闭液(PBS)	100ml	123.00元

碧云天生产的QuickBlock™封闭液(QuickBlock™ Blocking Buffer)是最新一代的快速高效封闭液，总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉、酪蛋白(Casein)等的封闭液及国外同类产品，可以用于Western blot (WB)、Immunofluorescence (IF)、Immunohistochemistry (IHC)、Immunocytochemitry (IC) 等实验中的封闭、一抗或二抗的稀释。本产品配制在PBS中，不含去垢剂。
QuickBlock™封闭液快速高效。封闭时间通常仅需5-15分钟，并且和BSA、脱脂奶粉、酪蛋白等传统封闭液以及国外同类的快速封闭液相比，显示出更强的信噪比(参考图1)。
QuickBlock™封闭液封闭后背景极低。本封闭液不含血清和白蛋白，确保极高的信噪比。
QuickBlock™封闭液兼容性好，兼容辣根过氧化物酶(Horseradish peroxidase, HRP)、碱性磷酸酶(Alkaline phosphatase, AP)和生物素标记的二抗。本产品中添加了不影响HRP和AP活性的防腐剂，不会干扰HRP或AP标记二抗的检测。同时本产品不含生物素，不会干扰基于生物素的检测。
QuickBlock™封闭液使用灵活。本产品配制在PBS中，可以直接用于相关实验，但也可以根据实验需要自行添加Tween-20或Triton X-100 至终浓度为0.05%-0.1%后用于封闭、一抗或二抗的稀释。
本产品与BSA及国外同类产品的封闭效果对比参见图1。在相同样品和实验条件下，仅封闭液及封闭时间存在如下图所示的差异时，碧云天的 QuickBlock™封闭液封闭后的整体背景明显低于BSA封闭后的背景，而且目的条带亮度明显高于国外同类品牌产品。

图1. QuickBlock™封闭液与BSA及国外同类产品的封闭效果比较。每组实验从左到右依次为：5μl蛋白Marker，2.5μg蛋白量的HeLa细胞裂解液，5μg蛋白量的HeLa细胞裂解液。请注意对于Antibody A，QuickBlock™封闭液比BSA和国外同类产品具有更低的背景，并且和国外同类产品相比信号明显更强。对于Antibody B，QuickBlock™封闭液比BSA具有更低的背景，和国外同类产品相比信号明显更强。实际实验结果会因样品、抗体、实验条件等的不同而存在差异，图中数据仅供参考。

关于不同封闭液的比较和选择，请参考碧云天的相关网页：http://www.beyotime.com/blocking- buffer.htm。
按照每张膜封闭需要5-10ml QuickBlock™封闭液计算，一个包装的本产品可以封闭10-20张膜。
包装清单：

产品编号	产品名称	包装
P0220	QuickBlock™封闭液(PBS)	100ml
—	说明书	1份

保存条件：
4℃保存，一年有效。长期不使用可以-20℃保存。
注意事项：
本产品推荐仅使用一次，重复使用可能会导致封闭效果下降。但对于一些信噪比很高的一抗，例如一些内参抗体，本封闭液可以重复使用2-3次。回收的封闭液请勿与未使用过的封闭液混合。
通常本产品用于PVDF膜及NC膜时的封闭时间为5-15分钟。对于一些背景非常高的抗体，可以尝试将封闭时间延长为30-60分钟。此外，如有特殊需要，也完全可以4℃封闭过夜。
由于没有任何一种封闭液是适用于所有实验体系的，因此对于一些特殊的实验，可能需要根据具体情况考虑使用其它更合适的封闭液。
取放PVDF膜和NC膜应使用平头镊子，并仅轻轻夹取其边角，操作过程须避免膜表面产生划痕、折痕或压痕等痕迹。
PVDF膜一经浸润和活化，需一直保持湿润，根据Western进行到的具体步骤可放置于western转膜液或洗涤液等适当溶液中，否则可能会产生难以封闭的异常背景。
在背景较高的情况下，为进一步提高信噪比，本产品中可以自行添加Tween-20或Triton X-100至终浓度为0.05%-0.1%。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. Western Blot中膜的封闭
a. 完成转膜后，用Western洗涤液洗涤蛋白膜1-2分钟。
b. 根据膜的大小，在平皿或者其它适当容器中倒入一定体积的QuickBlock™封闭液(PBS)(可以根据实验需要自行添加Tween-20或Triton X-100至终浓度为0.05%-0.1%)，确保封闭液后续能充分覆盖膜即可。对于常规的western，一张约6.6×8.5cm的膜推荐使用约10ml左右的封闭液。
c. 用平头镊夹住膜的一角，将膜放置在QuickBlock™封闭液(PBS)中，使封闭液完全浸没膜，置于水平摇床上封闭约10分钟(通常5-15分钟均可；经多种抗体的测试封闭10分钟的效果很多时候会显著优于常规的BSA封闭1小时的效果)。
d. 封闭后的膜即可用于一抗孵育等后续实验。详细的Western操作可以参考如下的相关网页：
http://www.beyotime.com/western.htm。
2. IF、IHC等实验的封闭
按照相关实验步骤，直接用QuickBlock™封闭液(PBS)替换传统封闭液即可(可以根据实验需要自行添加Tween-20或Triton X-100至终浓度为0.05%-0.1%)，一般封闭时间可以缩短至10分钟(在碧云天测试过的多个一抗中，封闭10-20分钟的效果无明显差别，封闭10分钟的效果等同于或显著优于常规的封闭方法)。

相关产品：

产品编号	产品名称	包装
P0220	QuickBlock™封闭液(PBS)	100ml
P0222	QuickBlock™封闭液(PBSTw)	100ml
P0226	QuickBlock™封闭液(PBSTx)	100ml
P0228	QuickBlock™封闭液(TBS)	100ml
P0231	QuickBlock™封闭液(TBSTw)	100ml
P0233	QuickBlock™封闭液(TBSTx)	100ml
P0235	QuickBlock™封闭液(10X)	100ml
P0252	QuickBlock™ Western封闭液	100ml
P0256	QuickBlock™ Western一抗稀释液	100ml
P0258	QuickBlock™ Western二抗稀释液	100ml
P0260	QuickBlock™免疫染色封闭液	100ml
P0262	QuickBlock™免疫染色一抗稀释液	100ml
P0265	QuickBlock™免疫荧光染色二抗稀释液	100ml
P0267	QuickBlock™免疫组化染色二抗稀释液	100ml

物质安全数据单

输入右侧验证码点击下载：

下载

质检证书

输入批号搜寻COA

搜索

相关产品请点击如下按钮：

Western检测

免疫染色

使用本产品的文献：

1. Ding M1, 2, 3, Ning J2, Feng N2, Li Z2, Liu Z2, Wang Y2, Wang Y2, Li X3, Huo C3, Jia X3, Xu R3, Fu F2, Wang X3, Pei J2.

J Pineal Res. 2018 Jan;64(1). doi: 10.1111/jpi.12447. (IF 14.528)

2. Zhao Z1, Zhao Y1, Zhuang XY2, Lo WC3, Baker MAB4, Lo CJ5, Bai F6.

Frequent pauses in Escherichia coli flagella elongation revealed by single cell real-time fluorescence imaging.

Nature Communications. 2018 May 14;9(1):1885. (IF 11.878)

3. Pei-PeiZhao, Hao-RanHu, Jia-YuLiu, Qin-FeiKe, Xiao-YuanPeng, HaoDing, Ya-PingGuo.

Gadolinium phosphate/chitosan scaffolds promote new bone regeneration via Smad/Runx2 pathway.

CHEM ENG J. 2019 March 1;359: 1120-1129. (IF 10.652)

4. Wali Muhammad, Jiaqi Zhu, Zihe Zhai, Jieqi Xie, Jiahang Zhou, Xudong Feng, Bing Feng, Qiaoling Pan, Shifen Li, Rajiu Venkatesan, Pan Li, Hongcui Cao, Changyou Gao

ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment

Acta Biomater. 2022 Aug:148:258-270. doi: 10.1016/j.actbio.2022.06.024. (IF 7.242)

5. Long J1, 2, Xiao Y3, Liu L4, Cao Y5, 6.

The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs.

J NANOBIOTECHNOL. 2017 Nov 10;15(1):80. (IF 6.518)

6. Hu H1, Zhao P2, Liu J2, Ke Q2, Zhang C1, Guo Y3, Ding H4.

Lanthanum phosphate/chitosan scaffolds enhance cytocompatibility and osteogenic efficiency via the Wnt/β-catenin pathway.

J NANOBIOTECHNOL. 2018 Nov 29;16(1):98. (IF 6.518)

7. Xingliang Tan, Zhenhua Liu, Yanjun Wang, Zhiming Wu, Yuantao Zou, Sihao Luo, Yi Tang, Dong Chen, Gangjun Yuan, Kai Yao

miR-138-5p-mediated HOXD11 promotes cell invasion and metastasis by activating the FN1/MMP2/MMP9 pathway and predicts poor prognosis in penile squamous cell carcinoma

Cell Death Dis. 2022 Sep 23;13(9):816. doi: 10.1038/s41419-022-05261-2. (IF 6.304)

8. Chen GL1, 2, Ye T1, Chen HL3, Zhao ZY4, Tang WQ4, Wang LS3, Xia JL1, 4.

Xanthine dehydrogenase downregulation promotes TGFβ signaling and cancer stem cell-related gene expression in hepatocellular carcinoma.

Oncogenesis. 2017 Sep 25;6(9):e382. (IF 6.119)

9. Chen GL, Ye T, Chen HL, Zhao ZY, Tang WQ, Wang LS, Xia JL.

Xanthine dehydrogenase downregulation promotes TGFβ signaling and cancer stem cell-relatedgene expression in hepatocellular carcinoma.

Oncogenesis. 2017 Sep 25;6(9):e382. (IF 6.119)

10. Fu C, Yin D, Nie H, Sun D.

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132.

CELL PHYSIOL BIOCHEM. 2018;51(4):1739-1750. (IF 5.5)

11. Tengfei Liu, Xiaoyan Liu, Hui Xiong, Cheng Xu, Jianxu Yao, Xiumei Zhu, Jianping Zhou and Jing Yao.

Mechanisms of TPGS and its derivatives inhibiting P-glycoprotein efflux pump and application for reversing multidrug resistance in hepatocellular carcinoma.

POLYM CHEM-UK. 2018;9:1827-1839. (IF 5.342)

12. Wei Chen, Wenjing Yang, Chunyan Zhang, Te Liu, Jie Zhu, Hao Wang, Tong Li, Anli Jin, Lin Ding, Jingrong Xian, Tongtong Tian, Baishen Pan, Wei Guo, Beili Wang

Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10

Oxid Med Cell Longev. 2022 Nov 24:2022:6986445. doi: 10.1155/2022/6986445. (IF 5.076)

13. Cheng L1, Zhang D2, Chen B

Tumor necrosis factor α-induced protein-3 protects zinc transporter 8 against proinflammatory cytokine-induced downregulation.

Exp Ther Med. 2016 Sep;12(3):1509-1514. (IF 1.785)

14. Peng W, Liu YJ, Hu MB, Yan D, Gao YX, Wu CJ.

Usingthe"target constituent removal combined with bioactivity assay" strategy to investigate the optimumarecoline content in charred areca nut.

SCI REP-UK. 2017 Jan 5;7:40278. (IF 3.998)

15. Chen J1, 2, Mao S2, Li H2, Zheng M1, Yi L2, Lin JM2, Lin ZX3.

The pathological structure of the perivascular niche in different microvascular patterns of glioblastoma.

PLoS One. 2017 Aug 3;12(8):e0182183. (IF 2.74)

16. Liang Y, Niu H, Ma L, Du D, Wen L, Xia Q, Huang W.

Eriodictyol 7 O β D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis.

Mol Med Rep. 2017 Aug;16(2):1298-1306. (IF 2.1)

17. Ding Y1, Zheng Y1, Liu T1, Chen T1, Wang C1, Sun Q1, Hua M2, Hua T3.

Changes in GABAergic markers accompany degradation of neuronal function in the primary visual cortex of senescent rats.

SCI REP-UK. 2017 Nov 2;7(1):14897. (IF 3.998)

18. Yao Y, Huang Y, Qian D, Zhang S, Chen Y, Bai B.

Effect of Various Ratios of Co-Cultured ATDC5 Cells and Chondrocytes on the Expression of Cartilaginous Phenotype in Microcavitary Alginate Hydrogel.

J Cell Biochem. 2017 Nov;118(11):3607-3615. (IF 4.237)

19. Li L, Zhao R, Li Y, Wang WH.

Antitumor activity of 2 [(2E) 3,7 dimethyl 2,6 octadienyl] 6 methyl 2,5 cyclohexadiene 1,4 dione isolated from the aerial part of Atractylodes macrocephala in hepatocellular carcinoma.

Mol Med Rep. 2017 Nov;16(5):6299-6305. (IF 2.1)

20. Zhai L, Li Y, Lan X, Ai L.

MicroRNA-10a-5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF.

Exp Ther Med. 2017 Dec;14(6):6147-6151. (IF 1.785)

21. Yang D1, Sun C1, Zhang J2, Lin S2, Zhao L2, Wang L1, Lin R1, Lv J1, Xin S1.

Proliferation of vascular smooth muscle cells under inflammation is regulated by NF-κB p65/microRNA-17/RB pathway activation.

Int J Mol Med. 2018 Jan;41(1):43-50. (IF 3.098)

22. Liu X1, Wang Z2, Zhang G3, Zhu Q3, Zeng H3, Wang T3, Gao F3, Qi Z3, Zhang J4, Wang R3.

Overexpression of asparaginyl endopeptidase is significant for esophageal carcinoma metastasis and predicts poor patient prognosis.

Oncol Lett. 2018 Jan;15(1):1229-1235. (IF 2.311)

23. Liu X, Wang Z, Zhang G, Zhu Q, Zeng H, Wang T, Gao F, Qi Z, Zhang J, Wang R.

Overexpression of asparaginyl endopeptidase is significant for esophageal carcinomametastasis and predicts poor patient prognosis.

Oncol Lett. 2018 Jan;15(1):1229-1235. (IF 2.311)

24. Li D, Ji H, Zhao B, Xu C, Xia W, Han L, Yu D, Ju Y, Jin C.

Therapeutic effect of ulinastatin on pulmonary fibrosis via downregulation of TGF β1, TNF α and NF κB.

Mol Med Rep. 2018 Jan;17(1):1717-1723. (IF 2.1)

25. Wang X1, Zou P1, He Y1, Meng K1, Quan F2, Zhang Y3.

Effect of luteinizing hormone on goat theca cell apoptosis and steroidogenesis through activation of the PI3K/AKT pathway.

Anim Reprod Sci. 2018 Mar;190:108-118. (IF 1.66)

26. Zhang L1, Liu J1, Zhou F1, Wang W1, Chen N1.

PGC-1α ameliorates kidney fibrosis in mice with diabetic kidney disease through an antioxidative mechanism.

Mol Med Rep. 2018 Mar;17(3):4490-4498. (IF 2.1)

27. Liu L1, Chang X1, Zhang Y1, Wu C1, Li R2, Tang L3, Zhou Z4.

Fluorochloridone induces primary cultured Sertoli cells apoptosis: Involvement of ROS and intracellular calcium ions-mediated ERK1/2 activation.

Toxicol In Vitro. 2018 Mar;47:228-237. (IF 2.959)

28. Chen G1, Li Q1, Luo Y2, Liu T2, Zhou S1, Pan E3, Peng L3.

Effect of Notoginsenoside R1 on autologous adipose graft in rats.

MOL MED REP. 2018 Apr;17(4):5928-5933. (IF 2.1)

29. Guo D1, Hu X2, Zhang H3, Lu C4, Cui G5, Luo X6.

Orientin and neuropathic pain in rats with spinal nerve ligation.

Int Immunopharmacol. 2018 May;58:72-79. (IF 3.943)

30. Song Z1, Yang F1, Du H1, Li X1, Liu J1, Dong M1, Xu X2, 3.

Role of artemin in non‐small cell lung cancer.

Thorac Cancer. 2018 May;9(5):555-562. (IF 2.61)

31. Yang HW1, Liu XY2, Shen ZF1, Yao W1, Gong XB2, Huang HX3, Ding GH1.

An investigation of the distribution and location of mast cells affected by the stiffness of substrates as a mechanical niche.

Int J Biol Sci. 2018 Jun 22;14(9):1142-1152. (IF 4.858)

32. Sun H1, Shao W2, Liu H2, Jiang Z3.

Exposure to 2,4-dichlorophenoxyacetic acid induced PPARβ-dependent disruption of glucose metabolism in HepG2 cells.

ENVIRON SCI POLLUT R. 2018 Jun;25(17):17050-17057. (IF 3.056)

33. Xu H1, Chen J1, Si X1, Chen M1, Pei F1, Qiu C1, Wu J1, Guan X1.

PKR inhibition mediates endotoxin tolerance in macrophages through inactivation of PI3K/AKT signaling.

Mol Med Rep. 2018 Jun;17(6):8548-8556. (IF 2.1)

34. Gao S1, Wang Y2, Li D1, Guo Y1, Zhu M3, Xu S1, Mao J1, Fan G4.

TanshinoneIIA Alleviates Inflammatory Response and Directs Macrophage Polarization in Lipopolysaccharide-Stimulated RAW264.7 Cells.

Inflammation. 2018 Sep 14. doi: 10.1007/s10753-018-0891-7. (IF 3.212)

35. Liu L1, Zhang Y1, Chang X1, Li R2, Wu C1, Tang L3, Zhou Z4.

Fluorochloridone perturbs blood-testis barrier/Sertoli cell barrier function through Arp3-mediated F-actin disruption.

Toxicol Lett. 2018 Oct 1;295:277-287. (IF 3.569)

36. Jin X1, Yu Y1, Zou Q2, Wang M1, Cui Y1, Xie J1, Wang Z1.

MicroRNA‐105 promotes epithelial‐mesenchymal transition of nonsmall lung cancer cells through upregulating Mcl‐1.

J Cell Biochem. 2018 Oct 14. doi: 10.1002/jcb.27873. (IF 4.237)

37. Wang Z1, Zhou W1, Dong H1, Ma X1, He Z1.

Dexmedetomidine pretreatment inhibits cerebral ischemia/reperfusion‑induced neuroinflammation via activation of AMPK.

Mol Med Rep. 2018 Oct;18(4):3957-3964. (IF 2.1)

38. Zhang W1, Chen W1, Li Z1, Ma L1, Yu J1, Wang H1, Liu Z1, Xu B1.

Identification and Characterization of Three New Cytochrome P450 Genes and the Use of RNA Interference to Evaluate Their Roles in Antioxidant Defense in Apis cerana cerana Fabricius.

Front Physiol. 2018 Nov 15;9:1608. (IF 3.367)

39. Ding Y1, 2, Chen T1, Wang Q1, Yuan Y1, Hua T1.

Axon initial segment plasticity accompanies enhanced excitation of visual cortical neurons in aged rats.

Neuroreport. 2018 Dec 12;29(18):1537-1543. (IF 1.394)

40. Lin L1, Wang L1, Liu Y1, Xu C1, Tu Y2, Zhou J1.

Non‑thermal plasma inhibits tumor growth and proliferation and enhances the sensitivity to radiation in vitro and in vivo.

Oncology Reports. Oncol Rep. 2018 Dec;40(6):3405-3415. (IF 3.041)

41. Shi GH1, Zhou L2.

Emodin suppresses angiogenesis and metastasis in anaplastic thyroid cancer by affecting TRAF6‑mediated pathways in vivo and in vitro.

Mol Med Rep. 2018 Dec;18(6):5191-5197. (IF 2.1)

42. Liang X, Li B, Huang Q, Liu D, Ma H.

Klotho prevents DEX-induced apoptosis in MC3T3-E1 osteoblasts through the NF-κB signaling pathway.

BIOCHEM BIOPH RES CO. 2018 Dec 9;507(1-4):355-361. (IF 2.985)

43. Wu JX1, Tong L1, Hu L1, Xia CM1, 2, Li M1, Chen QH3, Chen FX1, Du DS4.

Upregulation of Nav1.6 expression in the rostral ventrolateral medulla of stress-induced hypertensive rats.

Hypertens Res. 2018 Dec;41(12):1013-1022. (IF 2.941)

44. Wu K1, Mu XY2, Jiang JT2, Tan MY2, Wang RJ2, Zhou WJ3, Wang X2, He YY4, Li MQ5, Liu ZH2.

miRNA‑26a‑5p and miR‑26b‑5p inhibit the proliferation of bladder cancer cells by regulating PDCD10.

Oncol Rep. 2018 Dec;40(6):3523-3532. (IF 3.417)

45. Li XH1, Wang HP1, Tan J1, Wu YD1, Yang M1, Mao CZ2, Gao SF2, Li H2, Chen H3, Cai WB4.

Loss of pigment epithelium-derived factor leads to ovarian oxidative damage accompanied by diminished ovarian reserve in mice.

Life Sci. 2019 Jan 1;216:129-139. (IF 3.647)

46. Jin X, Yu Y, Zou Q, Wang M, Cui Y, Xie J, Wang Z

MicroRNA-105 promotes epithelial-mesenchymal transition of nonsmall lung cancer cells through upregulating Mcl-1.

J Cell Biochem. 2019 Apr;120(4):5880-5888. (IF 4.237)

47. Bei-Bei Deng, Bao-Ping Jiao, Yang-Jun Liu, Yan-Rong Li, Gui-Jun Wang

BIX-01294 enhanced chemotherapy effect in gastric cancer by inducing GSDME-mediated pyroptosis

Cell Biol Int. 2020 Sep;44(9):1890-1899. doi: 10.1002/cbin.11395. (IF 2.571)

48. Hongyang Liu, Siwei Xu, Guihua Li, Dayuan Lou, Xiaodan Fu, Qin Lu, Liman Hao, Jingsi Zhang, Jiajie Mei, Zheng Sui, Yu Lou

Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E-deficient mice

Exp Ther Med. 2020 Dec;20(6):170. doi: 10.3892/etm.2020.9300. (IF 1.785)

49. Ran Li, Fengying Gong, Hui Pan, Hanting Liang, Hui Miao, Yuxing Zhao, Lian Duan, Hongbo Yang, Linjie Wang, Shi Chen, Huijuan Zhu

Identification and In Vitro Functional Verification of Two Novel Mutations of GHR Gene in the Chinese Children with Laron Syndrome

Front Endocrinol (Lausanne). 2021 Apr 12;12:605736. doi: 10.3389/fendo.2021.605736.

50. Zhong Zheng, Xinyi Zheng, Yiwen Zhu, Zhixian Yao, Weiguang Zhao, Youjia Zhu, Feng Sun, Xingyu Mu, Yong Wang, Wanqing He, Zhihong Liu, Ke Wu, Junhua Zheng

IL-6 Promotes the Proliferation and Immunosuppressive Function of Myeloid-Derived Suppressor Cells via the MAPK Signaling Pathway in Bladder Cancer

Biomed Res Int. 2021 Apr 23;2021:5535578. doi: 10.1155/2021/5535578. (IF 2.276)

51. Yang Li, Dan-Yun Lai, Sheng-Ce Tao

SARS-CoV-2 spike linear epitope scanning via a peptide microarray through sera profiling

STAR Protoc. 2021 Sep 17;2(3):100707. doi: 10.1016/j.xpro.2021.100707.

52. Fangping Chen, Qing Liang, Lijie Mao, Yanrong Yin, Lixin Zhang, Cuidi Li, Changsheng Liu

Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis

Bioact Mater. 2021 Sep 10:10:335-344. doi: 10.1016/j.bioactmat.2021.09.001.

53. Jintao Chen, Sifeng Mao, Ziyi He, Lijuan Yang, Jinfeng Zhang, Jin-Ming Lin, Zhi-Xiong Lin

Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma

J Histochem Cytochem. 2022 Jan;70(1):99-110. doi: 10.1369/00221554211058098. (IF 2.187)

54. Jing Zhao, Qiuxia Duan, Cuihong Dong, Jing Cui

Cul4a attenuates LPS-induced acute kidney injury via blocking NF-kB signaling pathway in sepsis

J Med Biochem. 2022 Feb 2;41(1):62-70. doi: 10.5937/jomb0-33096. (IF 2.0)

55. Shengnan Zhang, Feihu Yan, Dongping Liu, Entao Li, Na Feng, Shengnan Xu, Hualei Wang, Yuwei Gao, Songtao Yang, Yongkun Zhao, Xianzhu Xia

Bacterium-Like Particles Displaying the Rift Valley Fever Virus Gn Head Protein Induces Efficacious Immune Responses in Immunized Mice

Front Microbiol. 2022 Mar 17:13:799942. doi: 10.3389/fmicb.2022.799942. (IF 4.235)

56. Dejun Xu, Xiaohan Jiang, Yukun Wang, Shuaifei Song

Liver Receptor homolog-1 Regulates Apoptosis of Bovine Ovarian Granulosa Cells by Progestogen Receptor Signaling Pathway

Animals (Basel). 2022 May 8;12(9):1213. doi: 10.3390/ani12091213.

57. Qingyuan Liu, Robert M Hoffman, Jing Song, Shiqi Miao, Jindong Zhang, Degang Ding, Delin Wang

Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma

Anticancer Res. 2022 May;42(5):2341-2354. doi: 10.21873/anticanres.15713. (IF 1.994)

58. Ping Wan, Taiyuan Li, Longfei Zhou, Jun Zhang, Xuefeng Rao

Eukaryotic Translation Initiation Factor 5A Independently Predicts Poor Prognosis of Cholangiocarcinoma Patients and Regulates the Ferroptosis and Mitochondrial Apoptosis

J Oncol. 2022 Jul 15:2022:4250531. doi: 10.1155/2022/4250531. (IF 2.206)

59. Wan-Hang Zhou, Yao Wang, Cong Yan, Wei-Dong Du, Maged Ali Al-Aroomi, Li Zheng, Shan-Feng Lin, Jia-Xing Gao, Sheng Jiang, Zeng-Xu Wang, Chang-Fu Sun, Fa-Yu Liu

CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma

Discov Oncol. 2022 Jul 29;13(1):67. doi: 10.1007/s12672-022-00533-x.

60. Qi Li, Baijian Jiang, Zhiqiang Zhang, Yongxiong Huang, Zhou Xu, Xinjin Chen, Jia Cai, Yu Huang, Jichang Jian

CRP Involved in Nile Tilapia ( Oreochromis niloticus) against Bacterial Infection

Biology (Basel). 2022 Jul 30;11(8):1149. doi: 10.3390/biology11081149.

61. Yi Liu, Zhao Lv, Tiaoyi Xiao, Xuewen Zhang, Chunhua Ding, Beibei Qin, Baohong Xu, Qiaolin Liu

Functional and Expressional Analyses Reveal the Distinct Role of Complement Factor I in Regulating Complement System Activation during GCRV Infection in Ctenopharyngodon idella

Int J Mol Sci. 2022 Sep 26;23(19):11369. doi: 10.3390/ijms231911369. (IF 4.556)

62. Hong Chen, Lingling Gu, Min Zhang, Huifen Chen, Hong Liao, Xueping Cao, Lu Yu, Jun Zhang

Interaction of miR-200a-3p with YAP regulates cell proliferation and metastasis differentially in HPV-positive and HPV-negative cervical cancer cells

BMC Cancer. 2022 Oct 4;22(1):1039. doi: 10.1186/s12885-022-10118-0. (IF 3.15)

63. Quan He, Wen Zhang, Jinjuan Zhang, Yuanyou Deng