碧云天生物技术-胰酶细胞消化液(0.05%胰酶)(C0202)

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胰酶细胞消化液(0.05%胰酶)

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产品编号: C0202

产品包装:100ml

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价格: ¥ 38.00

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产品编号	产品名称	产品包装	产品价格
C0202	胰酶细胞消化液(0.05%胰酶)	100ml	38.00元

碧云天生产的胰酶细胞消化液(Trypsin-EDTA Solution)含0.05%胰酶和0.02%EDTA，pH值为7.2-7.8。该消化液经过过滤除菌，可以直接用于培养细胞的消化，或者一些组织的消化。
本胰酶细胞消化液具有方便快速的特点，通常室温消化1分钟左右就可以消化下大多数贴壁细胞。
包装清单：

产品编号	产品名称	包装
C0202	胰酶细胞消化液(0.05%胰酶)	100ml
—	说明书	1份

保存条件：
4℃保存，一年有效。短期内不使用，推荐-20℃保存，-20℃可以保存更长时间。
注意事项：
在使用胰酶细胞消化液的过程中要特别注意避免消化液被细菌污染。
胰酶细胞消化液消化细胞时间不宜过长，否则细胞铺板后生长状况会较差。
本产品不宜整体回复至室温或37℃预热，室温存放或37℃加热会导致本产品酶活性下降。如果需要预热本产品后使用，宜仅取出所需使用的量进行预热。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 贴壁细胞的消化：
a.吸去培养液，用无菌的PBS、Hanks液或无血清培养液洗涤细胞一次，以去除残余的血清。
b.加入少量胰酶细胞消化液，略盖过细胞即可，室温放置30秒至2分钟。不同的细胞消化时间有所不同。
c.显微镜下观察，细胞明显收缩，并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化；或者用枪吹打细胞发现细胞刚好可以被吹打下来。此时吸除胰酶细胞消化液。加入含血清的完全细胞培养液，吹打下细胞，即可直接用于后续实验。
d.如果发现消化不足，则加入胰酶细胞消化液重新消化。
如果发现细胞消化时间过长，未及吹打细胞，细胞已经有部分直接从培养器皿底部脱落，直接用胰酶细胞培养液把细胞全部吹打下来。1000-2000g离心1分钟，沉淀细胞，尽量去除胰酶细胞消化液后，加入含血清的完全培养液重新悬浮细胞，即可用于后续实验。
2. 组织的消化：
a.不同的组织需要消化的时间相差很大，通常以消化后可以充分打散组织为宜。

附录：不同胰酶细胞消化液的比较和选择
1. 如果希望消化能力比较强，推荐选择C0201胰酶细胞消化液(0.25%胰酶)和C0203 胰酶细胞消化液(0.25%胰酶，含酚红)，这两种胰酶细胞消化液都含有EDTA，消化能力相对更强一些。
2. 如果希望观察比较方便，推荐选择含酚红的C0203 胰酶细胞消化液(0.25%胰酶，含酚红)和C0207 胰酶细胞消化液(0.25%胰酶，含酚红，不含EDTA)。
3. 对于酚红可能会干扰后续的测试分析，推荐选择不含酚红的C0201 胰酶细胞消化液(0.25%胰酶)和C0205 胰酶细胞消化液(0.25%胰酶，不含EDTA)。
4. 对于EDTA可能会干扰后续的测试分析时，推荐选择不含EDTA的C0205 胰酶细胞消化液(0.25%胰酶，不含EDTA)和C0207 胰酶细胞消化液(0.25%胰酶，含酚红，不含EDTA)。
5. 对于胰酶特别敏感的细胞，即对于消化时间特别快、消化时间比较难控制的情况，推荐选择C0202胰酶细胞消化液(0.05%胰酶)或C0204 胰酶细胞消化液(0.05%胰酶, 含酚红)。

相关产品：

产品编号	产品名称	包装
C0201	胰酶细胞消化液(0.25%胰酶)	100ml
C0202	胰酶细胞消化液(0.05%胰酶)	100ml
C0203	胰酶细胞消化液(0.25%胰酶, 含酚红)	100ml
C0204	胰酶细胞消化液(0.05%胰酶, 含酚红)	100ml
C0205	胰酶细胞消化液(0.25%胰酶, 不含EDTA)	100ml
C0207	胰酶细胞消化液(0.25%胰酶, 含酚红，不含EDTA)	100ml

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