化学信息：
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化学名
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1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl]imidazole;hydrochloride
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简称
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SKF96365
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别名
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SK and F 96365, SK and F-96365, SKF-96365, SKF 96365, SKF 96365 HYDROCHLORIDE
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中文名
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N/A
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化学式
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C22H26N2O3.HCl
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分子量
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402.91
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CAS号
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130495-35-1
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纯度
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98%
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溶剂/溶解度
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Water80mg/ml; DMSO80mg/ml; Ethanol80mg/ml
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溶液配制
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5mg加入1.24ml DMSO，或者每4.03mg加入1ml DMSO，配制成10mM溶液。SF1159-10mM用DMSO配制。
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生物信息：
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产品描述
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SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.
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信号通路
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Others
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靶点
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TRPC
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－
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－
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－
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－
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IC50
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－
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－
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－
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－
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－
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体外研究
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SKF-96365 exhibits protective activity against MPP+ injury in PC12 cells and significantly inhibits apoptotic cell death in PC12 cells after MPP+ administration. SKF-96365 does not exert effects on necrotic cell death induced by MPP+ insult in PC12 cells. Because of its non-selective activity, SKF-96365 has been demonstrated to have effects on multiple other Ca2+ channels: it not only blocks high-voltage-activated (HVA) Ca2+ channels at typically utilized test concentrations, but also potently inhibits low-voltage -activated (LVA) T-type Ca2+ channels in HEK293 cells. The exact effect of SKF-96365 on intracellular calcium homeostasis might dependent on cell types and experimental models used.
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体内研究
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SKF-96365 treatment inhibited the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade in vitro and in vivo.
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临床实验
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N/A
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特征
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N/A
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相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
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酶活性检测实验
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方法
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N/A
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细胞实验
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细胞系
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PC12 cells
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浓度
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1μM, 10μM, 50μM
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处理时间
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30 min
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方法
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To investigate whether SKF-96365 could protect PC12 cells from injury induced by MPP+ insult, cultured PC12 cells are pretreated with SKF-96365 in different concentrations (1μM, 10μM or 50μM) 30 min before MPP+ addition. The cells viability is measured 24h after MPP+ insult by using the cell proliferation reagent WST-1.
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动物实验
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动物模型
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C57BL/6N mice
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配制
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0.9% sterile saline
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剂量
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0.02μMol-0.2μMol/2μl/mouse
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给药方式
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intrastriatally
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参考文献：
1. Singh A, et al. Br J Pharmacol. 2010, 160(6):1464-1475.
2. Chen T, et al. PLoS One. 2013, 8(1):e55601.
3. Yao H, et al. Cell Death Differ. 2009, 16(12):1681-1693.
4. Jing Z, et al. Cancer Lett. 2016, 372(2):226-38.
包装清单：
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产品编号
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产品名称
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包装
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SF1159-10mM
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SKF96365 (TRPC抑制剂)
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10mM×0.2ml
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SF1159-5mg
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SKF96365 (TRPC抑制剂)
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5mg
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SF1159-25mg
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SKF96365 (TRPC抑制剂)
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25mg
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—
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说明书
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1份
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保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
注意事项：
本产品对人体有刺激性，操作时请小心，并注意适当防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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