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多肽与蛋白> 重组蛋白> 细胞因子
Recombinant Murine sTNF RII/TNFRSF1B
产品编号: P6065-100μg
产品包装:100μg
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价格: ¥ 7363.00
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产品编号 产品名称 产品包装 产品价格
P6065-5μg Recombinant Murine sTNF RII/TNFRSF1B 5μg 669.00元
P6065-100μg Recombinant Murine sTNF RII/TNFRSF1B 100μg 7363.00元
P6065-500μg Recombinant Murine sTNF RII/TNFRSF1B 500μg 12990.00元
P6065-1mg Recombinant Murine sTNF RII/TNFRSF1B 1mg -
Species Gene ID Accession Source Length MW Tag
Mouse 21938 P25119 E. coli 236aa 25.3kDa
About this Antibody
Name Recombinant Murine sTNF RII/TNFRSF1B (Recombinant Murine soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B; rMusTNF RII/TNFRSF1B);Recombinant Mouse sTNF RII/TNFRSF1B ; 重组小鼠可溶性肿瘤坏死因子受体II型/TNFRSF1B
Synonyms CD120b; TNFRSF1B; CD120b; CD120b antigen; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNF-RII; TNF-R-II; TNFR-II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Purity >97% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 2μg/ml, corresponding to a specific activity of >500IU/mg in the presence of 0.1ng/ml of rMuTNF-α.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin Less than 0.1EU/μg of rMusTNF RII/TNFRSF1B as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background TNFRSF1B also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is encoded by the TNFRSF1B gene in humans. It is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B has been shown to interact with TRAF2 and TTRAP. Recombinant murine TNFRSF1B is a 25.3kDa protein with 236 amino acid residues.
Amino Acid Sequence VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGG

包装清单:

产品编号 产品名称 包装
P6065-5μg Recombinant Murine sTNF RII/TNFRSF1B 5μg
P6065-100μg Recombinant Murine sTNF RII/TNFRSF1B 100μg
P6065-500μg Recombinant Murine sTNF RII/TNFRSF1B 500μg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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