微信在线咨询
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5331-500μg | Recombinant Human TNF-β/TNFSF1 | 500μg | 10830.00元 |
| P5331-10μg | Recombinant Human TNF-β/TNFSF1 | 10μg | 669.00元 |
| P5331-100μg | Recombinant Human TNF-β/TNFSF1 | 100μg | 6138.00元 |
| P5331-1mg | Recombinant Human TNF-β/TNFSF1 | 1mg | - |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 4049 | P01374 | E. coli | 171aa | 18.7kDa | － |
| About this protein | |
| Name | Recombinant Human TNF-β/TNFSF1 (Recombinant Human Tumor Necrosis Factor-β/TNFSF1; rHuTNF-β/TNFSF1)；重组人肿瘤坏死因子β/TNFS1 |
| Synonyms | LTA; Lymphotoxin alpha; tnfb; TNF-beta; TNFSF1; TNFSF1B; LT; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); TNFBlymphotoxin-alpha; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1 |
| Purity | >96% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5pg/ml, corresponding to a specific activity of >2.0×108IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20. |
| Endotoxin | Less than 0.1EU/μg of rHuTNF-β/TNFSF1 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNFbeta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1. |
| Amino Acid Sequence | LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5331-10μg | Recombinant Human TNF-β/TNFSF1 | 10μg |
| P5331-100μg | Recombinant Human TNF-β/TNFSF1 | 100μg |
| P5331-500μg | Recombinant Human TNF-β/TNFSF1 | 500μg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
微信在线咨询
