碧云天生物技术-SDS-PAGE蛋白上样缓冲液(2X)(P0015B)

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SDS-PAGE蛋白上样缓冲液(2X)

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产品编号: P0015B

产品包装:5ml

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价格: ¥ 38.00

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产品编号	产品名称	产品包装	产品价格
P0015B	SDS-PAGE蛋白上样缓冲液(2X)	5ml	38.00元

SDS-PAGE蛋白上样缓冲液(SDS-PAGE Sample Loading Buffer，2X)，是一种经过改良的以溴酚蓝为染料，2倍浓缩的蛋白上样缓冲液。
可以用于常规的SDS-PAGE蛋白样品的上样。
包装清单：

产品编号	产品名称	包装
P0015B	SDS-PAGE蛋白上样缓冲液(2X)	5ml
—	说明书	1份

保存条件：
-20℃保存，至少一年有效。
注意事项：
SDS-PAGE蛋白上样缓冲液(2X)中含少量DTT，有轻微刺激性气味，但不含剧毒的巯基乙醇。
SDS-PAGE蛋白上样缓冲液(2X)必须完全溶解后再使用。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 在室温或不超过37℃的水浴中溶解SDS-PAGE上样缓冲液(2X)。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。
2. 按照每1微升蛋白样品加入1微升蛋白上样缓冲液(2X)的比例，混合蛋白样品和蛋白上样缓冲液(2X)。
3. 100℃或沸水浴加热3-5分钟，以充分变性蛋白。
注意：如果起始时细胞或组织的用量较大，基因组DNA含量较高，煮沸3-5分钟后有可能仍然比较粘稠或者有粘稠状的半透明物体。此时需要再煮沸5-10分钟或者加入适量稀释成1X的蛋白上样缓冲液后再煮沸3-5分钟。充分煮沸后一方面可以使结合在基因组DNA上的蛋白充分释放，同时会导致基因组DNA的部分断裂从而使粘稠感消失，这样就不会影响后续的上样操作了。
4. 冷却到室温后，直接上样到SDS-PAGE胶加样孔内即可。
5. 通常电泳至蓝色染料到达胶的底端处附近即可停止电泳。

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