碧云天生物技术-SDS-PAGE凝胶配制试剂盒(P0012A)

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SDS-PAGE凝胶配制试剂盒

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产品编号: P0012A

产品包装:可制30-50块胶

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可制30-50块胶

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价格: ¥ 163.00

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产品编号	产品名称	产品包装	产品价格
P0012A	SDS-PAGE凝胶配制试剂盒	可制30-50块胶	163.00元

碧云天生产的SDS-PAGE凝胶配制试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶(即聚丙烯酰胺凝胶)。
SDS-PAGE凝胶配制试剂盒不仅可用于配制SDS-PAGE凝胶，也可用于配制非变性(native)PAGE凝胶。
本试剂盒约可配制30-50块常规大小的PAGE胶。
包装清单：

产品编号	产品名称	包装
P0012A-1	30% Acr-Bis (29:1)	100ml
P0012A-2	1M Tris-HCl, pH8.8	100ml
P0012A-3	10% SDS	5ml
P0012A-4	凝胶聚合催化剂	0.5g
P0012A-5	TEMED Substitute	0.5ml
P0012A-6	1M Tris-HCl, pH6.8	15ml
—	说明书	1份

保存条件：
1M Tris-HCl,pH8.8、10% SDS、凝胶聚合催化剂和1M Tris-HCl,pH6.8室温保存。30% Acr-Bis(29:1)和TEMED Substitute 4℃避光保存。凝胶聚合催化剂加水配制成10%溶液后，分装成小管-20℃保存，通常半年内有效。
注意事项：
凝胶聚合催化剂配制成10%溶液后，应当-20℃保存。同时应尽量减少室温存放时间，以防失效。
TEMED Substitute易挥发，使用后请盖紧瓶盖。另外凝胶凝聚的速度和温度及光照关系密切，可通过适当调节凝胶聚合催化剂和TEMED Substitute的用量，控制在不同的室内环境下凝胶凝聚的速度。
TEMED Substitute易燃，有腐蚀性，操作时请小心，并注意有效防护以避免直接接触人体或腐蚀其他物品。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.10%凝胶聚合催化剂的配制：例如称取0.1g凝胶聚合催化剂，用水溶解，并定容至1ml，即为10%凝胶聚合催化剂。
2.根据目的蛋白的分子量大小选择合适的凝胶浓度，再按照下面的表格配制SDS-PAGE的分离胶(即下层胶)：
不同浓度的SDS-PAGE分离胶的最佳分离范围：

SDS-PAGE分离胶浓度	最佳分离范围
6%胶	50-150kD
8%胶	30-90kD
10%胶	20-80kD
12%胶	12-60kD
15%胶	10-40kD

成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
6%胶	5	10	15	20	30	50
蒸馏水	2.0	4.0	6.0	8.0	12.0	20.0
30%Acr-Bis(29:1)	1.0	2.0	3.0	4.0	6.0	10.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED Substitute	0.004	0.008	0.012	0.016	0.024	0.04
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
8%胶	5	10	15	20	30	50
蒸馏水	1.7	3.3	5	6.7	10.0	16.7
30%Acr-Bis(29:1)	1.3	2.7	4.0	5.3	8.0	13.3
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED Substitute	0.003	0.006	0.009	0.012	0.018	0.03
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
10%胶	5	10	15	20	30	50
蒸馏水	1.3	2.7	4.0	5.3	8.0	13.3
30%Acr-Bis(29:1)	1.7	3.3	5.0	6.7	10.0	16.7
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED Substitute	0.002	0.004	0.006	0.008	0.012	0.02
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
12%胶	5	10	15	20	30	50
蒸馏水	1.0	2.0	3.0	4.0	6.0	10.0
30%Acr-Bis(29:1)	2.0	4.0	6.0	8.0	12.0	20.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED Substitute	0.002	0.004	0.006	0.008	0.012	0.02
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
15%胶	5	10	15	20	30	50
蒸馏水	0.5	1.0	1.5	2.0	3.0	5.0
30%Acr-Bis(29:1)	2.5	5.0	7.5	10.0	15.0	25.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED Substitute	0.002	0.004	0.006	0.008	0.012	0.02

注：如果配制非变性胶，参考上述配方，不加10%SDS即可配制成非变性PAGE胶。
3.按照如下表格配制SDS-PAGE的浓缩胶(也称堆积胶、积层胶或上层胶)：

成分	配制不同体积SDS-PAGE浓缩胶所需各成分的体积(毫升)
5%胶	2	3	4	6	8	10
蒸馏水	1.4	2.1	2.7	4.1	5.5	6.8
30%Acr-Bis(29:1)	0.33	0.5	0.67	1.0	1.3	1.7
1M Tris, pH6.8	0.25	0.38	0.5	0.75	1.0	1.25
10%SDS	0.02	0.03	0.04	0.06	0.08	0.1
10%凝胶聚合催化剂	0.02	0.03	0.04	0.06	0.08	0.1
TEMED Substitute	0.002	0.003	0.004	0.006	0.008	0.01

注：通常10-30分钟内胶会凝固。注：具体的凝固时间和温度及光照有关，说明书中10%凝胶聚合催化剂和TEMED Substitute的正常推荐用量是室温为25℃时的推荐用量。为达到与25℃时相近的凝固时间，当室温低于25℃时，可以适当同时加大10%凝胶聚合催化剂和TEMED Substitute的用量，例如20℃时建议用量是正常推荐用量的1.5倍，15℃时建议用量是正常推荐用量的2倍。

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Stroke Vasc Neurol. 2017 Apr 21;2(2):47-52.

54. Wang F, Lv P, Gu Y, Li L, Ge X, Guo G.

Galectin-1 knockdown improves drug sensitivity of breast cancer by reducing P-glycoprotein expression through inhibiting the Raf-1/AP-1 signaling pathway.

ONCOTARGET. 2017 Apr 11;8(15):25097-25106. (IF 5.168)

55. Cui C, Geng L, Shi J, Zhu Y, Yang G, Wang Z, Wang J, Chen M.

Structural and electrophysiological dysfunctions due to increased endoplasmic reticulum stress in a long-term pacing model using human induced pluripotent stem cell-derived ventricular cardiomyocytes.

Stem Cell Res Ther. 2017 May 11;8(1):109. (IF 5.116)

56. Chen JR, Jia XH, Wang H, Yi YJ, Li YJ.

With no interaction, knockdown of Apollon and MDR1 reverse the multidrug resistance of human chronic myelogenous leukemia K562/ADM cells.

Oncol Rep. 2017 May;37(5):2735-2742. (IF 3.417)

57. Mu J, Zhu D, Shen Z, Ning S, Liu Y, Chen J, Li Y, Li Z.

The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells.

BMC Cancer. 2017 May 2;17(1):307. (IF 3.15)

58. Wang W, Zhang H, Tang M, Liu L, Zhou Z, Zhang S, Wang L.

MicroRNA-592 targets IGF-1R to suppress cellular proliferation, migration and invasion in hepatocellular carcinoma.

Oncol Lett. 2017 May;13(5):3522-3528. (IF 2.311)

59. Ding Y, Zhang C, Zhang J, Zhang N, Li T, Fang J, Zhang Y, Zuo F, Tao Z, Tang S, Zhu W, Chen H, Sun X.

miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression.

Int J Oncol. 2017 May;50(5):1701-1710. (IF 3.899)

60. Liu C, Wang M, Sun W, Cai F, Geng S, Su X, Shi Y.

PU.1 serves a critical role in the innate defense against Aspergillus fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages.

Mol Med Rep. 2017 Jun;15(6):4084-4092. (IF 2.1)

61. Wang N, Zhang Q, Ning B, Luo L, Fang Y.

β-Asarone promotes Temozolomide's entry into glioma cells and decreases the expression of P-glycoprotein and MDR1.

Biomed Pharmacother. 2017 Jun;90:368-374. (IF 4.545)

62. Lin XL, Hu HJ, Liu YB, Hu XM, Fan XJ, Zou WW, Pan YQ, Zhou WQ, Peng MW, Gu CH.

Allicin induces the upregulation of ABCA1 expression via PPARγ/LXRα signaling in THP-1 macrophage-derived foam cells.

Int J Mol Med. 2017 Jun;39(6):1452-1460. (IF 3.098)

63. Guo X, Xiang Y, Yang H, Yu L, Peng X, Guo R.

Association of the LOX-1 rs1050283 Polymorphism with Risk for Atherosclerotic Cerebral Infarction and its Effect on sLOX-1 and LOX-1 Expression in a Chinese Population.

J Atheroscler Thromb. 2017 Jun 1;24(6):572-582. (IF 3.876)

64. Yu Y, Lv F, Liang D, Yang Q, Zhang B, Lin H, Wang X, Qian G, Xu J, You W.

HOTAIR may regulate proliferation, apoptosis, migration and invasion of MCF-7 cells through regulating the P53/Akt/JNK signaling pathway.

Biomed Pharmacother. 2017 Jun;90:555-561. (IF 4.545)

65. Sun R, Zhu G, Wang J, Tong L, Zhai J.

Indirect effects of X-irradiation on proliferation and osteogenic potential of bone marrow mesenchymal stem cells in a local irradiated rat model.

Mol Med Rep. 2017 Jun;15(6):3706-3714. (IF 2.1)

66. Wang Q, Ding W, Ding Y, Ma J, Qian Z, Shao J, Li Y.

Homoharringtonine suppresses imatinib resistance via the Bcl-6/p53 pathway in chronic myeloid leukemia cell lines.

ONCOTARGET. 2017 Jun 6;8(23):37594-37604. (IF 5.168)

67. Du L, Chen X, Duan Z, Liu C, Zeng R, Chen Q, Li M.

MiR-146a negatively regulates dectin-1-induced inflammatory responses.

ONCOTARGET. 2017 Jun 6;8(23):37355-37366. (IF 5.168)

68. Liu Z, Zeng W, Wang S, Zhao X, Guo Y, Yu P, Yin X, Liu C, Huang T.

A potential role for the Hippo pathway protein, YAP, in controlling proliferation, cell cycle progression, and autophagy in BCPAP and KI thyroid papillary carcinoma cells.

Am J Transl Res. 2017 Jul 15;9(7):3212-3223. (IF 3.375)

69. Yang M, Bai L, Yu W, Sun X, Xu G, Guan R, Yang Y, Qiu M, Zhang Y, Tian J, Fang H.

Expression of autophagy-associated proteins in papillary thyroid carcinoma.

Oncol Lett. 2017 Jul;14(1):411-415. (IF 2.311)

70. Huang Q, Liu X, Wu Y, Liao Y, Huang Y, Wei X, Ma M.

P38 MAPK pathway mediates cognitive damage in pentylenetetrazole-induced epilepsy via apoptosis cascade.

Epilepsy Res. 2017 Jul;133:89-92. (IF 2.208)

71. Zou Q, Tang Q, Pan Y, Wang X, Dong X, Liang Z, Huang D.

MicroRNA-22 inhibits cell growth and metastasis in breast cancer via targeting of SIRT1.

Exp Ther Med. 2017 Aug;14(2):1009-1016. (IF 1.785)

72. Cheng P, Wang T, Li W, Muhammad I, Wang H, Sun X, Yang Y, Li J, Xiao T, Zhang X.

Baicalin Alleviates Lipopolysaccharide-Induced Liver Inflammation in Chicken by Suppressing TLR4-Mediated NF-κB Pathway.

Front Pharmacol. 2017 Aug 18;8:547. (IF 4.225)

73. Xu H, Yuan Y, Wu W, Zhou M, Jiang Q, Niu L, Ji J, Liu N, Zhang L, Wang X.

Hypoxia stimulates invasion and migration of human cervical cancer cell lines HeLa/SiHa through the Rab11 trafficking of integrin αvβ3/FAK/PI3K pathway-mediated Rac1 activation.

J BIOSCI BIOENG. 2017 Sep;42(3):491-499. (IF 2.366)

74. Huang JL, Liu W, Tian LH, Chai TT, Liu Y, Zhang F, Fu HY, Zhou HR, Shen JZ.

Upregulation of long non-coding RNA MALAT-1 confers poor prognosis and influences cell proliferation and apoptosis in acute monocytic leukemia.

Oncol Rep. 2017 Sep;38(3):1353-1362. (IF 3.417)

75. Li H, Xiang Z, Liu Y, Xu B, Tang J.

MicroRNA-133b Inhibits Proliferation, Cellular Migration, and Invasion via Targeting LASP1 in Hepatocarcinoma Cells.

Oncol Res. 2017 Sep 21;25(8):1269-1282. (IF 4.949)

76. Yang M, Lv Y, Tian X, Lou J, An R, Zhang Q, Li M, Xu L, Dong Z.

Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro.

FRONT NEUROSCI-SWITZ. 2017 Oct 26;11:583. (IF 3.707)

77. Huang L, Deng M, Zhang S, Lu S, Gui X, Fang Y.

β-asarone and levodopa coadministration increases striatal levels of dopamine and levodopa and improves behavioral competence in Parkinson's rat by enhancing dopa decarboxylase activity.

Biomed Pharmacother. 2017 Oct;94:666-678. (IF 4.545)

78. Deng L, Liu T, Zhang B, Wu H, Zhao J, Chen J.

Forkhead box C1 is targeted by microRNA-133b and promotes cell proliferation and migration in osteosarcoma.

Exp Ther Med. 2017 Oct;14(4):2823-2830. (IF 1.785)

79. Wu X, Zhou H, Yue B, Li M, Liu F, Qiu C, Chen B, Ma X.

Upregulation of microRNA-25-3p inhibits proliferation, migration and invasion of osteosarcoma cells in vitro by directly targeting SOX4.

Mol Med Rep. 2017 Oct;16(4):4293-4300. (IF 2.1)

80. Zhao Y, Yang L, Huang Z, Lin L, Zheng G.

Synergistic effects of caffeine and catechins on lipid metabolism in chronically fed mice via the AMP-activated protein kinase signaling pathway.

Eur J Nutr. 2017 Oct;56(7):2309-2318. (IF 4.664)

81. Zhao M, Tang S, Xin J, Liu D.

Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia.

Exp Ther Med. 2017 Nov;14(5):4033-4040. (IF 1.785)

82. Fang D, He Y, Luan Z.

Simvastatin augments activation of liver regeneration through attenuating transforming growth factor-β1 induced-apoptosis in obstructive jaundice rats.

Exp Ther Med. 2017 Nov;14(5):4839-4845. (IF 1.785)

83. Du WL, Fang Q, Chen Y, Teng JW, Xiao YS, Xie P, Jin B, Wang JQ.

Effect of silencing the T-Box transcription factor TBX2 in prostate cancer PC3 and LNCaP cells.

Mol Med Rep. 2017 Nov;16(5):6050-6058. (IF 2.1)

84. Tao B, Yi J, Huang C, Xu W, Qin C, Chen L, Chen J, Gao Y, Wang R.

microRNA-96 regulates the proliferation of nucleus pulposus cells by targeting ARID2/AKT signaling.

Mol Med Rep. 2017 Nov;16(5):7553-7560. (IF 2.1)

85. Wang TT, Yuan JH, Ma JZ, Yang WJ, Liu XN, Yin YP, Liu Y, Pan W, Sun SH.

CTGF secreted by mesenchymal-like hepatocellular carcinoma cells plays a role in the polarization of macrophages in hepatocellular carcinoma progression.

Biomed Pharmacother. 2017 Nov;95:111-119. (IF 4.545)

86. Huang B, Tian Y, Zhang W, Ma Y, Yuan Y, Liu C.

Strontium doping promotes bioactivity of rhBMP-2 upon calcium phosphate cement via elevated recognition and expression of BMPR-IA.

COLLOID SURFACE B. 2017 Nov 1;159:684-695. (IF 4.389)

87. He X, Li T, Kang N, Zeng H, Ren S, Zong D, Li J, Cai S, Chen P, Chen Y.

The protective effect of PRMT6 overexpression on cigarette smoke extract-induced murine emphysema model.

INT J CHRONIC OBSTR. 2017 Nov 3;12:3245-3254. (IF 2.772)

88. Liang B, Xiao T, Long J, Liu M, Li Z, Liu S, Yang J.

Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy.

Int J Mol Med. 2017 Dec;40(6):1781-1791. (IF 3.098)

89. Lin Y, Wang X, Yu Y, Liu W, Xie F, Ouyang X, Huang Q.

Expression and prognostic significance of cyclin-dependent kinase inhibitor 1A in patients with resected gastric adenocarcinoma.

Oncol Lett. 2017 Dec;14(6):7473-7482. (IF 2.311)

90. Zhang C, Yu P, Zhu L, Zhao Q, Lu X, Bo S.

Blockade of α7 nicotinic acetylcholine receptors inhibit nicotine-induced tumor growth and vimentin expression in non-small cell lung cancer through MEK/ERK signaling way.

Oncol Rep. 2017 Dec;38(6):3309-3318. (IF 3.417)

91. Chang M, Wu M, Li H.

Curcumin combined with glycyrrhetinic acid inhibits the development of hepatocellular carcinoma cells by down-regulating the PTEN/PI3K/AKT signalling pathway.

Am J Transl Res. 2017 Dec 15;9(12):5567-5575. (IF 3.375)

92. Li E, Zhao Z, Ma B, Zhang J.

Long noncoding RNA HOTAIR promotes the proliferation and metastasis of osteosarcoma cells through the AKT/mTOR signaling pathway.

Exp Ther Med. 2017 Dec;14(6):5321-5328. (IF 1.785)

93. Huang W, Cheng P, Yu K, Han Y, Song M, Li Y.

Hyperforin attenuates aluminum-induced Aβ production and Tau phosphorylation via regulating Akt/GSK-3β signaling pathway in PC12 cells.

Biomed Pharmacother. 2017 Dec;96:1-6. (IF 4.545)

94. Jiao S, Liu Y, Yao Y, Teng J.

miR-124 promotes proliferation and differentiation of neuronal stem cells through inactivating Notch pathway.

Cell Biosci. 2017 Dec 11;7:68. (IF 5.026)

95. Wen L, Jiang X, Sun J, Li X, Li X, Tian L, Li Y, Bai W.

Cyanidin-3-O-glucoside promotes the biosynthesis of progesterone through the protection of mitochondrial function in Pb-exposed rat leydig cells.

Food Chem Toxicol. 2018 Feb;112:427-434. (IF 4.679)

96. Wang LM, Zhao N, Zhang J, Sun QF, Yang CZ, Yang PS.

Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway.

J Periodontal Res. 2018 Feb;53(1):66-72. (IF 2.926)

97. Pu T, Liu Y, Xu R, Li F, Chen S, Sun K.

Identification of ZFPM2 mutations in sporadic conotruncal heart defect patients.

Mol Genet Genomics. 2018 Feb;293(1):217-223. (IF 2.797)

98. Ouyang G, Xiong L, Liu Z, Lam B, Bui B, Ma L, Chen X, Zhou P, Wang K, Zhang Z, Huang H, Miao X, Chen W, Wen Y.

Inhibition of autophagy potentiates the apoptosis-inducing effects of photodynamic therapy on human colon cancer cells.

PHOTODIAGN PHOTODYN. 2018 Mar;21:396-403. (IF 2.894)

99. Xia PP, Li LJ, Qi RD, Shi JJ, Ju WZ, Chen ML.

Electrical and histological remodeling of the pulmonary vein in 2K1C hypertensive rats: Indication of initiation and maintenance of atrial fibrillation.

Anatol J Cardiol. 2018 Mar;19(3):169-175. (IF 1.223)

100. Wang S, Zhao Z, Fan Y, Zhang M, Feng X, Lin J, Hu J, Cheng Z, Sun C, Liu T, Xiong Z, Yang Z, Wang H, Sun D

Mst1 inhibits Sirt3 expression and contributes to diabetic cardiomyopathy through inhibiting Parkin-dependent mitophagy.

BBA-MOL BASIS DIS. 2018 Apr 17. pii: S0925-4439(18)30133-9. (IF 4.352)

101. Cui S, Sun Y, Liu Y, Liu C, Wang J, Hao G, Sun Q.

[Corrigendum] MicroRNA-137 has a suppressive role in liver cancer via targeting EZH2.

Mol Med Rep. 2018 May;17(5):7460. (IF 2.1)

102. Liang H, Sun H, Yang J, Yi C

miR‑145‑5p reduces proliferation and migration of hepatocellular carcinoma by targeting KLF5.

Mol Med Rep. 2018 Jun;17(6):8332-8338. (IF 2.1)

103. Yu G, Chen J, Li S, Pu P, Huang W, Zhao Y, Peng X, Wang R, Lei H

RING finger protein 10 prevents neointimal hyperplasia by promoting apoptosis in vitro and in vivo.

Life Sci. 2018 Sep 1;208:325-332. (IF 3.647)

104. Feng Y, Guo X, Huang X, Wu M, Li X, Wu S, Luo X

Metformin reverses stem cell‑like HepG2 sphere formation and resistance to sorafenib by attenuating epithelial‑mesenchymal transformation.

Mol Med Rep. 2018 Oct;18(4):3866-3872. (IF 2.1)

105. Wang N, Han Y, Luo L, Zhang Q, Ning B, Fang Y

β-asarone induces cell apoptosis, inhibits cell proliferation and decreases migration and invasion of glioma cells.

Biomed Pharmacother. 2018 Oct;106:655-664. (IF 4.545)

106. Qiu X, Guo Q, Liu X, Luo H, Fan D, Deng Y, Cui H, Lu C, Zhang G, He X, Lu A

Pien Tze Huang Alleviates Relapsing-Remitting Experimental Autoimmune Encephalomyelitis Mice by Regulating Th1 and Th17 Cells.

Front Pharmacol. 2018 Oct 31;9:1237. (IF 4.225)

107. Xiao LY, Wang XR, Yang JW, Ye Y, Zhu W, Cao Y, Ma SM, Liu CZ

Acupuncture Prevents the Impairment of Hippocampal LTP Through β1-AR in Vascular Dementia Rats.

Mol Neurobiol. 2018 Oct 55(10):7677-7690. (IF 4.5)

108. Liu H, Li X, Yu WQ, Liu CX

Upregulated EFNB2 and EPHB4 promotes lung development in a nitrofen-induced congenital diaphragmatic hernia rat model.

Int J Mol Med. 2018 Nov;42(5):2373-2382. (IF 3.098)

109. Tian X, Wang F, Luo Y, Ma S, Zhang N, Sun Y, You C, Tang G, Li S, Gong Y, Xie C

Protective Role of Nuclear Factor-Erythroid 2-Related Factor 2 Against Radiation-Induced Lung Injury and Inflammation.

Front Oncol. 2018 Nov 23;8:542. (IF 4.848)

110. Li SS, Zhang MX, Wang Y, Wang W, Zhao CM, Sun XM, Dong GK, Li ZR, Yin WJ, Zhu B, Cai HX

Reduction of PGRN increased fibrosis during skin wound healing in mice.

Histol Histopathol. 2018 Dec 18:18076. (IF 2.021)

111. Gao Z, Zhao GS, Lv Y, Peng D, Tang X, Song H, Guo QN

Anoikis‑resistant human osteosarcoma cells display significant angiogenesis by activating the Src kinase‑mediated MAPK pathway.

Oncol Rep. 2019 Jan 41(1):235-245. (IF 3.417)

112. Zhou ZQ, Chen Y, Chai M, Tao R, Lei YH, Jia YQ, Shu J, Ren J, Li G, Wei WX, Han YD, Han Y

Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts.

Int J Mol Med. 2019 Feb 43(2):890-900. (IF 3.098)

113. Huang X, Han X, Huang Z, Yu M, Zhang Y, Fan Y, Xu B, Zhou K, Song L, Wang X, Lu C, Xia Y

Maternal pentachlorophenol exposure induces developmental toxicity mediated by autophagy on pregnancy mice.

ECOTOX ENVIRON SAFE. 2019 Mar 169:829-836. (IF 4.872)

114. Wang C, Ren YL, Zhai J, Zhou XY, Wu J

Down-regulated LAMA4 inhibits oxidative stress-induced apoptosis of retinal ganglion cells through the MAPK signaling pathway in rats with glaucoma.

Cell Cycle. 2019 May 18(9):932-948. (IF 3.699)

115. Xing Y, Ren S, Ai L, Sun W, Zhao Z, Jiang F, Zhu Y, Piao D

ZNF692 promotes colon adenocarcinoma cell growth and metastasis by activating the PI3K/AKT pathway.

Int J Oncol. 2019 May 54(5):1691-1703. (IF 3.899)

116. Shen Y, Chen Q, Li L

Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.

MOL CELL PROBE. 2019 Jun 45:57-64. (IF 1.951)

117. Liu AH, Chu M, Wang YP

Up-Regulation of Trem2 Inhibits Hippocampal Neuronal Apoptosis and Alleviates Oxidative Stress in Epilepsy via the PI3K/Akt Pathway in Mice.

Neurosci Bull. 2019 Jun 35(3):471-485. (IF 4.326)

118. Liu B, Zhang Q, Wu X, Fu Y, Wang H, Guan Y, Yang S, Liu Y, Cao W, Wang J

Effect of Bletilla striata on the Prevention of Postoperative Peritoneal Adhesions in Abrasion-Induced Rat Model.

EVID-BASED COMPL ALT. 2019 Jun 10 2019:9148754. (IF 1.813)

119. Yang YH, Liu TH, Zhang LD, Chen ZY, Huang XS

Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis.

EVID-BASED COMPL ALT. 2019 Jun 16 2019:7164916. (IF 1.813)

120. Zhuang L, Qu H, Cong J, Dai H, Liu X

MiR-181c affects estrogen-dependent endometrial carcinoma cell growth by targeting PTEN.

Endocr J. 2019 Jun 28 66(6):523-533. (IF 1.952)

121. Ji Wei Li, Li Wei, Zhijun Han, Zhong Chen

Mesenchymal Stromal Cells-Derived Exosomes Alleviate Ischemia/Reperfusion Injury in Mouse Lung by Transporting Anti-Apoptotic miR-21-5p

Eur J Pharmacol. 2019 Jun 5;852:68-76. doi: 10.1016/j.ejphar.2019.01.022. (IF 3.263)

122. Tian X, An R, Luo Y, Li M, Xu L, Dong Z

Tamibarotene Improves Hippocampus Injury Induced by Focal Cerebral Ischemia-Reperfusion via Modulating PI3K/Akt Pathway in Rats.

J STROKE CEREBROVASC . 2019 Jul 28(7):1832-1840. (IF 1.598)

123. Chen JX, Xu X, Zhang S

Silence of long noncoding RNA NEAT1 exerts suppressive effects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation.

IUBMB Life. 2019 Jul 71(7):956-968. (IF 3.244)

124. Zhou GF, Jiang YH, Ma DF, Wang YC, Yang JL, Chen JY, Chi CY, Han XW, Li ZY, Li X

Xiao-Qing-Long Tang Prevents Cardiomyocyte Hypertrophy, Fibrosis, and the Development of Heart Failure with Preserved Ejection Faction in Rats by Modulating the Composition of the Gut Microbiota.

Biomed Res Int. 2019 Jul 18 2019:9637479. (IF 2.276)

125. Cao J, Zhang C, Jiang GQ, Jin SJ, Gao ZH, Wang Q, Yu DC, Ke AW, Fan YQ, Li DW, Wang AQ, Bai DS

Expression of GLS1 in intrahepatic cholangiocarcinoma and its clinical significance.

Mol Med Rep. 2019 Aug 20(2):1915-1924. (IF 2.1)

126. Su J, Wang J, Luo J, Li H

Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation.

MOL CELL PROBE. 2019 Aug 46:101415. (IF 1.951)

127. Li S, Cao H, Shen D, Chen C, Xing S, Dou F, Jia Q

Efficacy of Shoushen granule on adenosine triphosphate binding cassette transporter A1, proprotein convertase subtilisin/kexin type 9 and toll-like receptor 4/nuclear factor kappa-B signaling pathway

J Tradit Chin Med. 2019 Aug 39(4):524-534. (IF 0.676)

128. Ni T, Gao F, Zhang J, Lin H, Luo H, Chi J, Guo H

Impaired autophagy mediates hyperhomocysteinemia-induced HA-VSMC phenotypic switching.

J Mol Histol. 2019 Aug 50(4):305-314. (IF 2.531)

129. Sun Y, Lei B, Huang Q

SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine Mo

MED SCI MONITOR. 2019 Aug 20 25:6244-6254. (IF 1.918)

130. Li Q, Liu B, Breitkopf-Heinlein K, Weng H, Jiang Q, Dong P, Dooley S, Xu K, Ding H

Adenovirus‑mediated overexpression of bone morphogenetic protein‑9 promotes methionine choline deficiency‑induced non‑alcoholic steatohepatitis in non‑obese mice.

Mol Med Rep. 2019 Sep 20(3):2743-2753. (IF 2.1)

131. Jia Q, Cao H, Shen D, Li S, Yan L, Chen C, Xing S, Dou F

Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1.

Int J Mol Med. 2019 Sep 44(3):893-902. (IF 3.098)

132. Ren L, Qi K, Zhang L, Bai Z, Ren C, Xu X, Zhang Z, Li X

Glutathione Might Attenuate Cadmium-Induced Liver Oxidative Stress and Hepatic Stellate Cell Activation.

Biol Trace Elem Res. 2019 Oct 191(2):443-452. (IF 2.639)

133. Gao J, Yin X, Yu X, Dai C, Zhou F

Long noncoding LINC01551 promotes hepatocellular carcinoma cell proliferation, migration, and invasion by acting as a competing endogenous RNA of microRNA-122-5p to regulate ADAM10 expression.

J Cell Biochem. 2019 Oct 120(10):16393-16407. (IF 4.237)

134. Cao H, Jia Q, Shen D, Yan L, Chen C, Xing S

Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE-/- mice.

Exp Ther Med. 2019 Oct 18(4):2451-2458. (IF 1.785)

135. Yan R, Jiang Y, Lai B, Lin Y, Wen J

The positive feedback loop FOXO3/CASC11/miR-498 promotes the tumorigenesis of non-small cell lung cancer.

BIOCHEM BIOPH RES CO. 2019 Nov 12 519(3):518-524. (IF 2.985)

136. Qin S, Wang H, Liu G, Mei H, Chen M

miR‑21‑5p ameliorates hyperoxic acute lung injury and decreases apoptosis of AEC II cells via PTEN/AKT signaling in rats.

Mol Med Rep. 2019 Dec 20(6):4953-4962. (IF 2.1)

137. Jia Q, Cao H, Shen D, Yan L, Chen C, Xing S

Fisetin, via CKIP-1/REGγ, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells.

Eur J Pharmacol. 2019 Dec 15 865:172748. (IF 3.263)

138. Cao H, Jia Q, Yan L, Chen C, Xing S, Shen D

Quercetin Suppresses the Progression of Atherosclerosis by Regulating MST1-Mediated Autophagy in ox-LDL-Induced RAW264.7 Macrophage Foam Cells.

Int J Mol Sci. 2019 Dec 3 20(23). pii: E6093. (IF 4.556)

139. Jie Luo, Huaiming Wang, Huanjie Chen, Guolian Gan, Yifeng Zheng

CLDN4 Silencing Promotes Proliferation and Reduces Chemotherapy Sensitivity of Gastric Cancer Cells Through Activation of the PI3K/Akt Signalling Pathway

Exp Physiol. 2019 Dec 19. doi: 10.1113/EP088112. (IF 2.431)

140. Shan-Shan Li, Hui Cao, Ding-Zhu Shen, Chuan Chen, San-Li Xing, Fang-Fang Dou, Qing-Ling Jia

Effect of Quercetin on Atherosclerosis Based on Expressions of ABCA1, LXR-α and PCSK9 in ApoE -/- Mice

Chin J Integr Med. 2020 Feb;26(2):114-121. doi: 10.1007/s11655-019-2942-9. (IF 1.545)

141. Wenfeng Xie, Lei Chen, Li Chen, Qiuye Kou

Silencing of long non-coding RNA MALAT1 suppresses inflammation in septic mice: role of microRNA-23a in the down-regulation of MCEMP1 expression

Inflamm Res. 2020 Feb;69(2):179-190. doi: 10.1007/s00011-019-01306-z. (IF 3.174)

142. Wang J, Zuo Y, Zhuang K, Luo K, Yan X, Li J, Zhang JH, Liu F

Recombinant Human Milk Fat Globule-Epidermal Growth Factor 8 Attenuates Microthrombosis after Subarachnoid Hemorrhage in Rats.

J STROKE CEREBROVASC . 2020 Mar 29(3):104536. (IF 1.598)

143. Xicheng Wang, Xining Wang, Yunxiuxiu Xu, Maolin Yan, Wenxin Li, Jie Chen, Tao Chen

Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation

Cancer Cell Int. 2020 Mar 24;20:91. doi: 10.1186/s12935-020-01172-4. (IF 4.175)

144. Yuanyuan Li, Jiapo Li, Yue Hou, Ling Huang, Yue Bian, Guiyu Song, Chong Qiao

Circadian clock gene Clock is involved in the pathogenesis of preeclampsia through hypoxia

Life Sci. 2020 Apr 15;247:117441. doi: 10.1016/j.lfs.2020.117441. (IF 3.647)

145. Yanjun Li, Ruimin Guo, Mengying Zhang, Peng Chen, Jingxin Li, Yanni Sun

Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture

Exp Ther Med. 2020 Jun;19(6):3521-3530. doi: 10.3892/etm.2020.8625. (IF 1.785)

146. Xidan Zhu, Jia Feng, Wenguang Fu, Xiaojia Shu, Xue Wan, Jinbo Liu

Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following β‑catenin silencing

Int J Mol Med. 2020 Jun;45(6):1838-1850. doi: 10.3892/ijmm.2020.4543. (IF 3.098)

147. Ping Wang, Jiming Xu, Weijing You, Yongfeng Hou, Shuiliang Wang, Yujie Ma, Jianming Tan, Zengli Zhang, Wentao Hu, Bingyan Li

Knockdown of CYP24A1 Aggravates 1α,25(OH) 2 D 3-Inhibited Migration and Invasion of Mouse Ovarian Epithelial Cells by Suppressing EMT

Front Oncol. 2020 Jul 29;10:1258. doi: 10.3389/fonc.2020.01258. (IF 4.848)

148. Bing Liu, Lihong Yan, Xuefei Jiao, Xiaozhi Sun, Zonggang Zhao, Junwei Yan, Mingjin Guo, Yunjin Zang

Lycopene Alleviates Hepatic Hypoxia/Reoxygenation Injury Through Nrf2/HO-1 Pathway in AML12 Cell

J Interferon Cytokine Res. 2020 Aug;40(8):406-417. doi: 10.1089/jir.2020.0038.

149. Kun Ma, Chuan Zhang, Wuyin Li

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150. Yuan Bian, Qiu Li, Qiaolian Li, Ruigen Pan

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154. Guangyu Qian, Xiaoyan Jin, Louwei Zhang

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156. Pengcheng Dou, Yu He, Bo Yu, Juan Duan

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158. Jing Huo, Taibin Liu, Fenghua Li, Xinfeng Song, Xiuli Hou

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159. Cheng Long, Shiqiang Cen, Zhou Zhong, Chang Zhou, Gang Zhong

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161. Qing Zhang, Ting Chen, Yun Zhang, Lingxia Lyu, Bohuan Zhang, Chengchen Huang, Xuhao Zhou, Yutao Wu, Zhoubin Li

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162. Chengwei Chen, Yaozeng Xu

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163. Chensheng Zhao, Fan Yang, Xiaona Wei, Jingwen Zhang

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164. Hui Jiang, Kefeng Cao, Chang Fan, Xiaoya Cui, Yanzhen Ma, Jian Liu

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165. Dezhi Chen, Min Zhang

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166. Yan Hao, Jie Miao, Wenjia Liu, Li Peng, Yue Chen, Qing Zhong

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167. Liangping Zhao, Li Wang, Daimin Zhang, Yuqi Chen, Fulu Jin

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168. Linyan Wang, Juan Zhang

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169. Yunlin Ge, Shuchen Ding, Jiangbiao Feng, Jiangdong Du, Zenghui Gu

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181. Xuan Ma, Yang Liu, Hao Tian, Bo Zhang, Meiling Wang, Xia Gao

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184. Huiming Guo, Jianyuan Gao, Yuan Qian, Huawei Wang, Jiang Liu, Qingyan Peng, Yong Zhou, Kunhua Wang

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185. Xingli Liu, Wei Song, Xiangmin Zhang, Fangmin Long, Junkun Yin, Xiao He, Liang Lv

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187. Kangjun Zhang, Taishi Fang, Yajie Shao, Yanhui Wu

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196. Yong Lin, Yushan Chen, Rongqiang Shen, Dingzhu Chen, Yimin Lin

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200. Xu Li, Xin Zhang, Yajun Liu, Ruihan Pan, Xiaolong Liang, Lifa Huang, Chao Yang

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201. Mingfang Huang, Xuqiang Liao, Liang Li, Gao Li, Minbiao Chen

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202. Huanhuan Zhu, Shaopeng Tong, Yan Cui, Xiaodong Wang, Minying Wang

Tanshinol alleviates ulcerative colitis by promoting the expression of VLDLR

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