中文 | English
订货电话
400-1683301
800-8283301
电话订货时间:
工作日9:00-17:00
订货邮件
order@beyotime.com
订货QQ
4001683301
微信在线咨询
订单下载
碧云天订单.xls
抗体> 其它类别一抗> Transcription Factors
HMGB1 Rabbit Polyclonal Antibody (KO Validated)
产品编号: AF5192
产品包装:50μl
选择包装
价格: ¥ 593.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF5192 HMGB1 Rabbit Polyclonal Antibody (KO Validated) 50μl 593.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Rabbit WB, IF, IHC H, M, R 24kDa 29kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 - 1:50-1:200 1:50-1:200 1:50-1:200 - - -

抗体详细信息如下::

About this Antibody
Name HMGB1 Rabbit Polyclonal Antibody (KO Validated)
Category Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype IgG
Purification method Affinity purification
Positive samples 293T, MCF7, Jurkat, Mouse spleen, Mouse lung, Mouse kidney, Rat lung
Cellular location Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Customer validation WB (Human, Mouse); IHC (Mouse, Rat); IF (Mouse)
About the Immunogen
Immunogen A synthetic peptide of human HMGB1 (NP_002119.1).
Sequence SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE
Gene ID 3146
Swiss Prot P09429
Synonyms HMGB1; HMG-1; HMG1; HMG3; SBP-1; high mobility group box 1
Category Transcription Factors; Epigenetics
Background High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein. HMGB2 is widely expressed during embryonic development, but is restricted to lymphoid organs and testis in adult animals. HMGB3 is only expressed during embryogenesis. While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix. HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 facilitates the binding of Hox proteins, Oct-1, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis, but not apoptosis. Extracellular HMGB1 "alarms" the innate immune system by acting as a chemoattractant for inflammatory leukocytes, smooth muscle cells, and stem cells, functioning as an immune adjuvant for soluble and particulate antigens, and triggering activation of T cells and dendritic cells. In addition, activated monocytes, macrophages and, dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. Hypoxia has also been shown to cause the release of HMGB1 in the liver, and some studies suggest a role for extracellular HMGB1 in tumor homeostasis.

包装清单:

产品编号 产品名称 包装
AF5192 HMGB1 Rabbit Polyclonal Antibody (KO Validated) 50μl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:
HMGB1 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:

Fig. 1. Western blot analysis of extracts of various cell lines, using HMGB1 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s. Fig. 2. Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 3. Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody at dilution of 1:100 (40x lens). Fig. 4. Immunofluorescence analysis of HeLa cells using HMGB1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

相关产品:

产品编号 产品名称 包装
E6001 MiniProGel™蛋白制胶与电泳系统(4胶) 1套
E6005 MiniProGel™蛋白制胶与电泳系统(2胶) 1套
E6050 MiniBlot™蛋白转膜系统 1套
E6150 MiniProGel™蛋白制胶、电泳与转膜系统(2胶/2膜) 1套
E6155 MiniProGel™蛋白制胶、电泳与转膜系统(4胶/2膜) 1套
E6159 MiniProGel™蛋白制胶、电泳与转膜系统(4胶/4膜) 1套
E6080 BeyoPower™中电流电源(300V/600mA/100W) 1套
E6085 BeyoPower™高电流电源(300V/2000mA/200W) 1套
E6600 TissueMaster™手持式组织研磨仪 1套
EI600 BeyoImager™ 600化学发光成像系统 1套
FFP24 PVDF膜(进口分装, 6.6×8.5cm, 0.2μm) 20张/包装
FFP32 PVDF膜(进口分装, 6.6×8.5cm, 0.45μm) 20张/包装
FFP51 转印滤纸(7.5×10cm) 100张/包装
FFX050-FFX055 Western洗膜盒 1个/包装
P0006 Bradford蛋白浓度测定试剂盒 1000次
P0012 BCA蛋白浓度测定试剂盒 500次
P0012A SDS-PAGE凝胶配制试剂盒 可制30-50块胶
P0012AC SDS-PAGE凝胶快速配制试剂盒 可制30-50块胶
P0013 Western及IP细胞裂解液 100ml
P0013B-P0013D RIPA裂解液(强、中、弱) 100ml
P0014A/P0014B SDS-PAGE电泳液(Tris-Gly, Powder) 1L/10L
P0014C/P0014D SDS-PAGE电泳液(Tris-Gly, 10X) 100ml/500ml
P0015/P0015L SDS-PAGE蛋白上样缓冲液(5X) 2ml/15ml
P0015F SDS-PAGE蛋白上样缓冲液(6X) 2ml
P0017F BeyoBlue™考马斯亮蓝超快染色液 250ml
P0018A BeyoECL Star (特超敏ECL化学发光试剂盒) 100ml/500ml
P0018F BeyoECL Moon (极超敏ECL化学发光试剂盒) 100ml/500ml
P0018S/P0018M BeyoECL Plus (超敏ECL化学发光试剂盒) 100ml/500ml
P0021A Western转膜液 1L
P0021S PVDF膜浸润活化液 250ml
P0022 丽春红染色液 120ml
P0023A Western一抗稀释液 100ml/500ml
P0023B Western封闭液 100ml/500ml
P0023C Western洗涤液 250ml
P0023D Western二抗稀释液 100ml/500ml
P0025 Western一抗二抗去除液 250ml
P0059 Recombinant 16-Tag Protein 200μl/1ml
P0066 预染蛋白质分子量标准(19-117kD) 200μl
P0068 彩色预染蛋白质分子量标准(10-180kD) 200μl
P0071 BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) 200μl
P0075 BeyoColor™彩色预染蛋白分子量标准(10-170kD) 200μl
P0078 BeyoColor™彩色预染蛋白分子量标准(15-120kD) 200μl
P0095 免疫染色通透液(Saponin) 100ml/500ml
P0096 免疫染色通透液(Triton X-100) 100ml/500ml
P0097 免疫染色强力通透液 100ml/500ml
P0098 免疫染色固定液 100ml/500ml
P0099 4%多聚甲醛固定液 100ml/500ml
P0102 免疫染色封闭液 100ml
P0103 免疫染色一抗稀释液 100ml
P0106 免疫染色洗涤液 250ml
P0108 免疫荧光染色二抗稀释液 100ml
P0220-P0235 QuickBlock™封闭液 100ml
P0252 QuickBlock™ Western封闭液 100ml/500ml
P0256 QuickBlock™ Western一抗稀释液 100ml/500ml
P0258 QuickBlock™ Western二抗稀释液 100ml/500ml
P0260 QuickBlock™免疫染色封闭液 100ml
P0262 QuickBlock™免疫染色一抗稀释液 100ml
P0265 QuickBlock™免疫荧光染色二抗稀释液 100ml
P0267 QuickBlock™免疫组化染色二抗稀释液 100ml
P0280 InstantView™ SDS-PAGE蛋白染色及上样缓冲液(5X) 1ml
P0050-P0058 BeyoGel™ SDS-PAGE预制胶(Tris-Gly, 12孔) 10块/50块
P0448-P0472 BeyoGel™ Plus PAGE预制胶(Tris-Gly, 10孔/15孔) 10块/50块
P0501-P0528 BeyoGel™ Plus PAGE预制胶(Hepes, 10孔/15孔) 10块/50块
P0552 BeyoGel™ Plus SDS-PAGE Hepes电泳液(20X) 100ml/500ml
P0670-P0678 SDS-PAGE下层胶预混液 250ml/500ml
P0683 SDS-PAGE上层胶预混液 250ml/500ml
P0686-P0695 SDS-PAGE凝胶超快速配制试剂盒 可制30-50块胶
P1005-P1031 蛋白酶抑制剂混合物(50X/100X) 1ml/5ml
P1045-P1066 蛋白酶磷酸酶抑制剂混合物(50X) 1ml/2ml/5ml/10ml
P1081-P1097 磷酸酶抑制剂混合物(50X) 2ml/10ml
P1112/P1113 去乙酰化酶抑制剂混合物(100X) 1ml/5ml
R0121 AllProtect™动物组织核酸、蛋白稳定保存液 25ml/100ml


物质安全数据单
输入右侧验证码点击下载:
更换校验码
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮:
使用本产品的文献:
购物车
会员
顶部