碧云天生物技术-Bax抗体(小鼠单抗)(AB026)

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Bax抗体(小鼠单抗)

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产品编号: AB026

产品包装:>20次

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价格: ¥ 732.00

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产品编号	产品名称	产品包装	产品价格
AB026	Bax抗体(小鼠单抗)	>20次	732.00元

来源	用途	交叉反应性	抗体识别位点	Bax分子量
Mouse	WB, IP, IC, IF, ELISA	H, M, R	Bax N-terminal	～21kD

WB,Western blot; IP,Immunoprecipitation; IC,Immunocytochemistry; IF,Immunofluorescence.
H,human;M,mouse;R,rat.
本Bax抗体(Bax antibody)为进口分装，以人工合成的Bax氨基端(Amino-terminal)的一段多肽为抗原制备而成的抗Bax小鼠单克隆抗体。克隆号为6A7(Clone6A7)，是应用最广的Bax抗体。抗体类型为Mouse IgG1。
本Bax抗体可以检测内源性水平的Bax，未发现和其它Bcl-2家族蛋白有交叉反应。
Bax是一种促凋亡蛋白，上调Bax蛋白水平可以促进细胞凋亡。在通过线粒体应激诱导的细胞凋亡中Bax起关键作用。Bax可以形成寡聚体，然后从细胞浆中转移到线粒体膜上；通过和线粒体膜上的一些pore proteins相互作用，增强线粒体的通透性(permeability)；最后导致细胞色素c释放(cytochrome c release)、caspase-9激活以及后续的其它caspase激活和最终的细胞凋亡。Bax可以和Bcl-2形成多聚体，来调节线粒体膜的通透性，最终调节细胞的凋亡或存活。
配套提供了Western一抗稀释液，可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例为(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IC	IF	ELISA
1:500-1000	1:50	1:500	1:500	1:500-5000

本抗体如果用于常规的Western检测，至少可以检测20次。
包装清单：

产品编号	产品名称	包装
AB026-1	Bax抗体(小鼠单抗)	20μl
AB026-2	Western一抗稀释液	20ml
—	说明书	1份

保存条件：
Bax抗体-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
对于本抗体，Western检测时一抗要4℃缓慢摇动过夜，如果仅短时间与一抗孵育检测效果较差。
在Western实验后，请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体，包括已经使用过的稀释抗体，4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.Western检测：
A.按照1:500-1000用碧云天提供的Western一抗稀释液稀释抗体。
B.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C.回收稀释的一抗，4℃保存以备下次继续使用。
D.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。

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