消化原理图
产品特点
- 消化能力强:比同类产品具有更强的消化能力,能对复杂样品中不同IgG进行快速消化。每毫升Pepsin Agarose沉淀中含有约2-3mg Pepsin,可以消化不少于40mg IgG;
- 稳定性高,底物特异性强:固定后的胃蛋白酶活性保持时间更长,孵育时间可延长至过夜。特异性切割蛋白中的芳香L-氨基酸肽键、抗体铰链区的重链等,获得的产物纯度高,适用于Western、ELISA、质谱等后续分析测试;
- 优质的基质材料:使用高品质的Beyorose™ 6FF琼脂糖作为基质,确保了更好的稳定性和高纯度,有效避免非特异性反应。该基质与Sepharose 6 Fast Flow性能基本一致;
- 使用便捷:消化IgG后可通过离心去除胃蛋白酶琼脂糖凝胶,停止酶切反应,省去了传统的离子交换等柱分离纯化步骤;
- 兼容性强:Western及IP细胞裂解液(P0013)、RIPA裂解液(P0013B/C/D)或NP-40裂解液(P0013F)等都完全兼容本产品。
工作流程
相关产品
产品编号 |
产品名称 |
包装 |
产品价格 |
P2015-2ml |
Protein A Agarose (Fast Flow, 抗体纯化用) |
2ml |
|
P2015-10ml |
Protein A Agarose (Fast Flow, 抗体纯化用) |
10ml |
|
P2015-50ml |
Protein A Agarose (Fast Flow, 抗体纯化用) |
50ml |
|
P2015-200ml |
Protein A Agarose (Fast Flow, 抗体纯化用) |
200ml |
|
P2017-2ml |
Protein G Agarose (Fast Flow, 抗体纯化用) |
2ml |
|
P2017-10ml |
Protein G Agarose (Fast Flow, 抗体纯化用) |
10ml |
|
P2017-50ml |
Protein G Agarose (Fast Flow, 抗体纯化用) |
50ml |
|
P2017-200ml |
Protein G Agarose (Fast Flow, 抗体纯化用) |
200ml |
|
P2019-2ml |
Protein A+G Agarose (Fast Flow, 抗体纯化用) |
2ml |
|
P2019-10ml |
Protein A+G Agarose (Fast Flow, 抗体纯化用) |
10ml |
|
P2019-50ml |
Protein A+G Agarose (Fast Flow, 抗体纯化用) |
50ml |
|
P2019-200ml |
Protein A+G Agarose (Fast Flow, 抗体纯化用) |
200ml |
|
P2049-1ml |
Pepsin agarose (胃蛋白酶琼脂糖凝胶) |
1ml |
|
P2049-5ml |
Pepsin agarose (胃蛋白酶琼脂糖凝胶) |
5ml |
|
P2049-20ml |
Pepsin agarose (胃蛋白酶琼脂糖凝胶) |
20ml |
|
P2157-1ml |
Biotin Agarose (生物素琼脂糖凝胶) |
1ml |
|
P2157-5ml |
Biotin Agarose (生物素琼脂糖凝胶) |
5ml |
|
P2157-20ml |
Biotin Agarose (生物素琼脂糖凝胶) |
20ml |
|
P2159-1ml |
Streptavidin Agarose (链霉亲和素琼脂糖凝胶) |
1ml |
|
P2159-5ml |
Streptavidin Agarose (链霉亲和素琼脂糖凝胶) |
5ml |
|
P2159-20ml |
Streptavidin Agarose (链霉亲和素琼脂糖凝胶) |
20ml |
|
P2165-1ml |
Heparin Agarose (肝素琼脂糖凝胶) |
1ml |
|
P2165-5ml |
Heparin Agarose (肝素琼脂糖凝胶) |
5ml |
|
P2165-20ml |
Heparin Agarose (肝素琼脂糖凝胶) |
20ml |
|
P2169-1ml |
Iodoacetyl Agarose (碘乙酰琼脂糖凝胶) |
1ml |
|
P2169-5ml |
Iodoacetyl Agarose (碘乙酰琼脂糖凝胶) |
5ml |
|
P2169-20ml |
Iodoacetyl Agarose (碘乙酰琼脂糖凝胶) |
20ml |
|
P2169-100ml |
Iodoacetyl Agarose (碘乙酰琼脂糖凝胶) |
100ml |
|